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{{/_source.additionalInfo}}Anti-BCLAF1 [M33-P5B11]
(Cat#: 151874)
Description
| Cat# | 151874 |
| Applications | ELISA, IHC, WB |
| Antigen/Gene or Protein Targets | BCL2 Associated Transcription Factor 1 (BCLAF1) |
| Reactivity | Human |
| Relevance | BCLAF1 was associated with poor prognosis and showed aberrant localization in colorectal cancer. |
| Host | Mouse |
| Immunogen | Peptide Sequence - KYQGDGIVED (amino acids 900 - 909) |
| Positive Control | ELISA- Peptide immunogenWestern Blot- Hela whole cell extractIHC- Human colon carcinoma |
| Subclass | IgG1 kappa |
| Recommended Growing Conditions | Dulbecco's media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine(Sigma G7513) + 3ml of Penicillin -Streptomycin (Sigma P0781). |
| Strain | Balb/c |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Epigenetics & Nuclear Signalling |
| References | The expression and prognostic significance of bcl-2-associated transcription factor 1 in rectal cancer following neoadjuvant therapy. Europe PMC ID: 26183150 |
Image: Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).
For Research Use Only
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