Cat# | 152858 |
Applications | ELISA, FACS, WB |
Antigen/Gene or Protein Targets | Interferon-gamma (IFNγ) |
Synonyms | IFG, IFI, IFN gamma, IFN, immune, IFN-gamma, IFNG, IFNG_HUMAN, Immune interferon, Interferon gamma |
Reactivity | Salmon and Rainbow Trout |
Relevance | The zebrafish genome contains ten genes that encode class II cytokine-like peptides, of which the two that are related most closely to mammalian interferon gamma (IFN-gamma) were named IFN-gamma1 and IFN-gamma2. |
Host | Mouse |
Positive Control |
Western blot: recombinant IFNγ and kidney lysates FACS: isolated leukocytes, kidney and spleen cells |
Subclass | Not known |
Myeloma Used | P3X63Ag8.653 |
Strain | Balb/c |
Research Area | Aquaculture |
Image: Recognition of trout IFN-γ within head kidney cells stimulated with trout rIL-2 in vitro. Samples were lysed in Tri-Reagent and RNA and protein were extracted for analysis. Results from qPCR (A) and WB (B) were correlated, and 72-h incubation of HK cells with rIL-2 showed a high level of IFN-γ.
For Research Use Only.
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