Cat# | 152862 |
Applications | ELISA, FACS, WB |
Antigen/Gene or Protein Targets | Interleukin 22 (IL22) |
Synonyms | Cytokine Zcyto18, IL 10 related T cell derived inducible factor, IL 21, IL 22, IL D110, IL TIF, IL-10-related T-cell-derived-inducible factor, IL-22, IL-TIF, IL21, Il22, ILD110, ILTIF |
Reactivity | Salmon and Rainbow Trout |
Relevance | IL-22 is an α-helical cytokine that binds to a heterodimeric cell surface receptor composed of IL-10R2 and IL-22R1 subunits. IL-22R is expressed on tissue cells, and it is absent on immune cells. |
Host | Mouse |
Immunogen | Ovalbumin-conjugated synthetic peptide sequence located within the central region of the mature IL-22 polypeptide |
Positive Control |
Western blot: recombinant IL22 (rIL22), gill lysates FACS: isolated leukocytes, kidney and spleen cells |
Subclass | IgG1 kappa |
Myeloma Used | P3X63Ag8.653 |
Strain | Balb/c |
Research Area | Aquaculture |
Image: Immunodetection of native IL-22 production within gill tissues after challenging with the bacterial fish pathogen Yersinia ruckeri. A: Relative expression of IL-22 in fish gill tissues as determined by qPCR. B: Immunodetection of IL-22 protein in the gill after purifying protein samples from Tri® Reagent. M: Molecular weight marker; +: Recombinant IL-22; -: Recombinant IL-2B. Fish number 1 exhibited low levels of IL-22 gene expression (A) and did not display detectable levels of IL-22 protein (B). Conversely, fish number 2 showed high levels of IL-22 gene expression (A) and produced detectable amounts of IL-22 protein (B). The MHC Class II antibody was used as a protein quality/loading control.
For Research Use Only.
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