Cat# | 153529 |
Applications | ELISA IHC WB |
Antigen/Gene or Protein Targets | IgT, Immunoglobulin Heavy Constant Tau |
Synonyms | IgT |
Reactivity | Gilthead seabream, Meagre and Lumpsucker |
Relevance | Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. This Anti-IgT antibody provides a useful tool to monitor vaccine performance in fish and will assist in the development of future vaccines, especially mucosal vaccines. Target species: Meagre (Argyrosomus regius), gilt-head (sea) bream (Sparus aurata), Lumpsucker (Cyclopteridae), Large yellow croaker (Larimichthys crocea). |
Host | Mouse |
Immunogen | Ovalbumin-conjugated synthetic peptide |
Positive Control |
ELISA: fish sera, peptide immunogen Western blot: sera IHC: formalin-fixed, paraffin-embedded head kidney, gill and spleen tissues |
Subclass | IgG |
Myeloma Used | P3X63Ag8.653 |
Notes | Recommended Usage Conditions: ELISA- neat; Western blot- neat; IHC- 1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 |
Research Area | Aquaculture |
Image: Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Positive membranous immunostaining is observed in Meagre gill tissue using Anti-IgT [Z55F8*C3]. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).
For Research Use Only.
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