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{{/_source.additionalInfo}}Patho Gene-spin DNA/RNA Extraction Kit
Cat#: 17154
Description
The Patho Gene-spin DNA/RNA Extraction Kit is designed for rapid and sensitive DNA/RNA isolation from pathogenic samples infected by viruses or bacteria such as plasma, blood, serum, cell-free body fluids, cells and tissue.
The included lysis buffer contains chaotropic salt that immediately inactivates DNase/RNase to ensure that the isolated DNA/RNA is intact. This results in a stronger lysis efficiency than conventional products. The columns provided in this kit have advanced silica-gel membrane technology for rapid and effective purification of DNA/RNA without organic extraction or ethanol precipitation. The buffering conditions are finely adjusted to provide optimum binding of the DNA/RNA to the column.
The purified RNA/DNA is free of proteins and nucleases and is suitable for use in downstream applications in pathogen detection.
- Stable extraction of purified DNA/RNA from various pathogenic samples
- High lysis efficiency without additional additives
- Uses silica-gel spin columns for rapid extraction without cross contamination
- Lysis buffer contains chaotropic salt that immediately inactivates DNase/RNase
- Viral nucleic acids are eluted in low elution volumes of 30-60 µl
- Does not require organic extraction or ethanol precipitation
- Simple protocol is completed in 30 minutes
- Pathogen detection
- cDNA synthesis
- Quantitative PCR (qPCR, qRT-PCR)
- PCR or RT-PCR
- Infectious disease research
- Lysis buffer, 35ml
- Binding buffer, 35ml
- Washing buffer A, 30ml
- Washing buffer B, 10ml
- Elution buffer, 20ml
- Spin columns x 50
Sufficient for 50 preps.
