Home New Customer? Create Account
Member Login:

DNA Polymerases/SNPase, dNTPs & Kits

108725/108750 - One Step Whole Genome Amplification Kit

PRICE

Starting at: $445.00

Please Choose:

Size



Add to Cart:
The One Step Whole Genome Amplification Kit is an improved version of the DOP (degenerated oligo PCR) with optimized primer sequences and our unique SD Polymerase. This enzyme combines the strand displacement capability with heat stability up to 92°C. Due to these features, it is possible to use this enzyme for both amplification steps without additional pipetting or handling.
 
The WGA Kit is designed for reproducible amplification of low copy number genomic DNA to yield 2-10 µg of product in about 2 hours. All components necessary for amplification of human genomic DNA from single cells or low copy templates are included.
 

Features

  • Amplification finished in less than 2 hours
  • Different genomic amplification coverage than other enzymes
  • Long fragments up to 3 Kb
  • One Enzyme for the whole genome amplification
  • Ready for PCR and NGS
  • Suitable for single cell DNA Analysis
 

Applications

  • Single cell DNA analysis
  • Next Generation Sequencing (NGS)
  • SNP Genotyping
  • qPCR- and PCR-based mutation detection
  • STR and RFLP analysis
  • Array technologies (comparative genomic hybridization)
 

Sizes

108725 (25 reactions)

  • 2.5x Master Mix without Enzyme, 250µl 
  • SD Polymerase, 10 U/µl, 25µl
  • PCR grade H2O, 1800µl
  • Human genomic DNA as positive control, 70µl

108750 (50 reactions)

  • 2.5x Master Mix without Enzyme, 500µl
  • SD Polymerase, 10 U/µl, 50µl
  • PCR grade H2O, 1800µl
  • Human genomic DNA as positive control, 70µl
 

Publications