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Culture Media

GCM07.0500 - Terrific Broth, 500g

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Terrific Broth is a dehydrated powder for the preparation of nutritionally rich liquid medium to be used with glycerol for the growth and maintenance of recombinant strains of E. coli in molecular biology studies.

This rich growth medium contains all the nutritional requirements for E. coli to support high cell density and maintain growth in the logarithmic phase for an extended period of time, resulting in larger yields of plasmid DNA and heterologous proteins. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth. The potassium phosphate buffer system acts to prevent cell death. Glycerol serves as an additional source for carbon as well as a source for carbohydrates. Glycerol is advantageous over glucose in that it is not fermented into acetic acid and, unlike glucose, it does not act as a repressor. 

 
Formulation (g/l)
  • Tryptone: 12.00
  • Yeast Extract: 24.00
  • K2HPO4: 12.54
  • KH2PO4: 2.31
  • Final pH (25°C): 7.0 ± 0.2
 

Features

  • Contains all the nutritional requirements for E. coli
  • Supports high cell density
  • Prolongs growth in the logarithmic phase, creating larger yields of plasmid DNA and heterologous proteins
  • Used with glycerol rather than glucose; unlike glucose, glycerol is not fermented into acetic acid and does not act as a repressor
 

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