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Immunoassay Optimization Buffers

100 000 - LowCross-Buffer®

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Antibody and sample diluent for minimizing non-specific binding, cross-reactivities and matrix effects in immunoassays.

LowCross-Buffer® by Candor Bioscience is an innovative assay buffer for ELISA, EIA, RIA, protein arrays, western blotting, immunohistochemistry and immuno-PCR. It is ready-to-use and can replace your standard sample or antibody dilution buffer.

The formulation of LowCross-Buffer® helps reduce immunoassay interference and can therefore greatly improve the quality of detection. The use of LowCross-Buffer® minimizes:

  • Non-specific binding of the antibodies
  • Negative effects of interfering substances
  • Low or medium affinity cross-reactivities of the antibodies
  • Matrix effects, such as those from blood sera or plasma specimens
  • Interference caused by HAMAs (Human Anti-Mouse Antibodies) and rheumatoid factors

Reduction of these negative effects improves the quality of the assay and the reliability of the results.

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Features

  • Improves quality of results and reliability of assay
  • Reduces cross-reactivities, matrix effects and non-specific binding
  • Can be used with both monoclonal and polyclonal antibodies 
  • Can be used in combination with fluorescent dyes
  • Free of phosphate
  • Can also be used as a washing buffer, especially in challenging or sensitive assays such as immuno-PCR
 

Applications

  • ELISA
  • Western Blotting
  • Lateral Flow Assays
  • Protein Arrays/Multiplex Assays
  • Immuno-PCR
  • Immunohistochemistry
  • Enzyme Immunoassay
  • Radioimmunoassay
 

Sizes

  • 100 050 – LowCross-Buffer®, 50ml
  • 100 125 – LowCross-Buffer®, 125ml
  • 100 500 – LowCross-Buffer®, 500ml
 

Literature

 

Publications

 

FAQs

1. What is special about LowCross-Buffer®?
LowCross-Buffer® is a sample buffer for immunoassays that minimizes unwanted interference, such as non-specific binding, cross-reactivities and matrix effects. The high affinity, specific binding of the antibody to the analyte is retained, while undesired binding of the antibody is prevented.

2. How does LowCross-Buffer® reduce matrix effects?
Matrix effects usually result from unwanted low or medium affinity binding of the matrix components, such as serum proteins from human serum specimen, to the analyte or antibody. If the analyte is masked by proteins or other components of the specimen, the antibody cannot bind to the analyte. Antibodies can be masked by matrix components as well. LowCross-Buffer® reduces this masking of antibodies.

3. How is LowCross-Buffer® used?
LowCross-Buffer® is a dilution buffer for specimens, such as serum or plasma, and/or for detection antibodies. Its use depends on the interference. If the non-specific binding or cross-reactivity derives from the detection antibody, we recommend diluting the detection antibody in LowCross-Buffer®.

4. Does LowCross-Buffer® need to be diluted?
No. LowCross-Buffer® is ready-to-use. Sample or detection antibodies are diluted directly with the buffer. In competitive assays and some applications of immunohistochemistry, it can be useful to dilute LowCross-Buffer® in water or physiological buffers. In this case, the user has to determine the ideal mixing ratio by testing.

5. Can buffer components influence the color reaction of enzymatic detection?
No. LowCross-Buffer® does not influence the enzymatic activity of alkaline phosphatase or of peroxidase negatively. Sometimes users detect an increase of enzymatic activity by incubating the labeled detection antibody in LowCross-Buffer®.

6. Can I use LowCross Buffer® in combination with fluorescent dyes, such as in protein arrays?
Yes. For protein arrays there are positive results. Using cyan dyes, such Cy5, Cy3, and Oyster-dyes, may increase fluorescence in some applications.

7. How often can I freeze and thaw LowCross-Buffer®?
LowCross-Buffer® can be frozen and thawed several times, but it is important to mix the buffer thoroughly before use.

8. Can LowCross-Buffer® replace a blocking solution for surfaces?
No. LowCross-Buffer® is not a blocking buffer for surfaces. It can be used for dilution of the specimens or antibodies. For blocking, we recommend The Blocking Solution

 

100000, 100050, 100-050, 100125, 100-125, 100500, 100-500, low-cross


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