Anti-Tet-Repressor Antibody

Positive control (Cat# TETR1)
tet-structure
Sales Price$187.00
Antibody 
Description

Our set of polyclonal and monoclonal Anti-Tet Repressor (TetR) antibodies, manufactured by MoBiTec, possess excellent binding properties and have been successfully tested for use in ELISA, western blot and immunofluorescence assays. 

The tetracycline regulatory system is widely used for selective target gene regulation in eukaryotic cells. These antibodies target the Tet-Repressor protein (TetR-B). TetR(B) has a length of 207 amino acids and a mass of 23,355 Da. The immunogen is TetR-tetO (Accession no. P04483).

Three antibodies are offered:

  • TET01 - rabbit polyclonal antibody; can be used in ELISA, western blot and immunofluorescence. 
  • TET02 - optimized mix of two different epitope-specific monoclonal mouse antibodies.
  • TET03 - single monoclonal mouse antibody; ideal for immunofluorescence microscopy.

TETR1, a purified 23 kDa Tet-Repressor (B) protein from E. coli transposon Tn10, is available as a positive control. 

 

  TET01 TET02 TET03
Type Rabbit polyclonal IgG Monoclonal IgG1, K mix  Monoclonal IgG1, K
Immunogen TetR(B)-tetO TetR(B)-tetO TetR(B)-tetO
Purification Affinity purified via Protein G columns Affinity purified via Protein A or G columns Affinity purified via Protein A or G columns
Epitope -- TetR(B): Amino acid #84-98, Amino acid #26-53 TetR(B): Amino acid #37-44
Reconstitution In: 200µl distilled water 100µl distilled water 100µl distilled water
Working Dilution for Immunofluorescence n.d. n.d. n.d.
Working Dilution for Western Blots and ELISA 1:1000 1:500 - 1:2000 1:1000
Detection Limit for ELISA 0.2 ng 20-50 pg n.d.
Detection Limit for Western Blot 0.8 ng  0.8 - 1.0 ng 5 ng
 
Please note: MoBiTec anti-TetR(B) antibodies were raised against TetR(B)-tetO, but are generally able to detect variants of TetR(B) as well, such as rtTA and its derivatives, whereby TET02 is the most sensitive and promising choice. However, in contrast to TetR(B), rtTA regulator proteins are required and present in cells in much smaller amounts, especially in stable cell lines. Additionally, the regulated gene and its product may downregulate these regulators further, so that it's even more obscured. Detection of rtTA and its derivatives is more difficult than that of TetR(B) and often fails at the level of Western blotting. In such cases, PCR may be used as surrogate due to its sensitivity. Nevertheless, independent references have proven the suitability of MoBiTec's anti-TetR(B) antibodies to detect rtTA. See examples below: 
 
Therefore, MoBiTec shall not be made responsible or liable for any claims or loss arising from the failure of the anti-TetR(B) antibodies to detect rtTA and its derivatives.
 
  • Prime tool for studying tet regulatory systems in eukaryotic cells 
  • Suited for ELISA, western blots and immunofluorescence (for cells only, not for tissues or tissue slices) 
  • Excellent binding properties
  • Western blotting
  • ELISA
  • Immunofluorescence
  • Detection of Tet-Repressor (TetR)
  • Detection of Tet-Repressor fusion protein (TetR-Fusion)
  • Detection of tetracycline responsive transactivator (tTA)
  • Detection of reverse tetracycline responsive transactivator (rtTA) such as rtTA-S or rtTA-M
Cat# Description Quantity
TET01 Anti-Tet-Repressor, Polyclonal Rabbit, Lyophilized 3mg
TET02 Anti-Tet-Repressor, Monoclonal IgG1, Mix, Lyophilized 1mg
TET03 Anti-Tet-Repressor, Monoclonal IgG1, Lyophilized 50µg
TETR1 TET-Repressor Protein, Positive Control 1µg
References with Tet-Repressor (TetR) Antibodies

 

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