MagSi-DNA Beads, 3µm Bead Size

Silica Surface, 2ml (Cat# MD01022)
mbeads-photo
Sales Price$203.00
Surface 
Amount 
Description

MagSi-DNA beads are magnetic silica beads with a nanoporous surface optimal for nucleic acid binding. Due to the superparamagnetic properties and bead size of 3µm, the beads sediment very slowly and typically collects within 1-2 minutes in a magnetic field. This makes them applicable for both manual and automated/robotic DNA isolation.

The magnetic microparticles are intended as a solid phase extraction tool for custom buffer systems based on chaotropic and non-chaotropic binding principles. They can be used for developing your own nucleic acid isolation and extraction methods, such as:

  • Isolation of genomic, mitochondrial, or viral DNA; isolation of RNA 
  • Isolation of genomic, plasmid or phage DNA from bacterial cultures and bacteria from clinical samples 
  • Clean-up of DNA from enzymatic reactions or chromatin immunoprecipitation procedures to remove unwanted substances

MagSi-DNA beads reversibly bind DNA and other nucleic acids under sample- and buffer-specific conditions. A solution containing DNA (e.g. lysate) is combined with the beads and an application-specific binding buffer. After incubation, nucleic acids are bound to the silica surface. By applying a suitable magnet to the container (tube/deepwell microplate), the bead pellet is separated from the sample mixture. Unwanted components are further removed by washing steps in a selection of buffers. Finally, nucleic acids are released in a DNase/RNase-free water or buffer solution.

MagSi-DNA beads are available with a silica surface and with a carboxylated surface. The two surfaces differ in their optimal binding conditions. When developing a new application, it is recommended to try both types in parallel.

Please note: When separation speed is crucial and sufficient homogenization tools are available, 300nm beads may be more suitable due to their short separation time.

 
  • Fast separation and short suspension time 
  • Optimized for nucleic acid binding
  • Can be used in both manual and automated/robotic DNA isolation
  • Concentration of 20mg/ml
  • Nucleic acid isolation and purification
  • Developing your own nucleic acid isolation and extraction methods
Cat# Product Surface Volume
MD01022 MagSi-DNA 3.0 Silica 2ml
MD03022 MagSi-DNA 3.0 Silica 10ml
MD01024 MagSi-DNA 3.0 COOH Carboxylated 2ml
MD03024 MagSi-DNA 3.0 COOH Carboxylated 10ml

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