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Automated Magnetic Bead-Based Kits for Purification of Nucleic Acids

MDKT00210096 - MagSi-NA Pathogens DNA/RNA Purification Kit, 96 Preps

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The MagSi-NA Pathogens kit is a magnetic-bead based kit for manual and automated isolation of DNA and RNA from serum, plasma, oropharyngeal and nasopharyngeal swabs, and any other type of respiratory samples. 

With ready-to-use reagents and a simple protocol, this kit provides fast, convenient extraction. The included MagSi-PA VII magnetic beads are optimized for fast separation even from viscous sample lysates. Purified total nucleic acids can be used for qPCR and other pathogen detection methods. This kit is ideal for automation as it does not require repeated centrifugation, vacuum filtration or column separation.

The purification procedure begins with a liquid sample incubated with the optimized Lysis Buffer PA1. The lysis incubation with shaking at room temperature is sufficient to release pathogen nucleic acids. Subsequently the MagSi-PA VII beads and Binding Buffer U1 are added. Following a binding incubation and magnetic separation, the MagSiPA VII beads are washed three times to remove inhibitors and contaminants. Finally, the purified total nucleic acids are eluted at room temperature, magnetic beads are removed and the purified fraction containing nucleic acids can be directly used for further pathogen detection.

 

Features

  • Suitable for small, medium and high-throughput automation
  • Used with blood samples, liquid samples (plasma, serum, urine, swab washes, etc.), tissue samples and feces
  • Short, simple protocol; small elution volumes
  • Strong magnetic beads enable fast magnetic separation even from viscous sample lysates
  • Purified total RNA/DNA is suitable for enzymatic down-stream applications 

 

Applications

  • DNA and RNA extraction

 

Contents

  • Lysis Buffer PA1, 20ml
  • Binding Buffer U1, 40ml
  • Wash Buffer I, 2 x 80ml
  • Wash Buffer II, 80ml
  • Elution Buffer, 20ml
  • Proteinase K, 20 mg 
  • Poly-A-RNA, 0.3mg
  • Poly-A-RNA Buffer, 0.5ml
  • MagSi-PA VII Beads, 2ml
  • Poly-A-RNA Buffer, 10 x 0.5ml
  • MagSi-PA VII Beads, 20ml

 

Literature