MagSi-WCX Beads with Weak Cation Exchange

2ml (Cat# MD01023)
mbeads-photo
Sales Price$961.00
Size 
Description

MagSi-WCX magnetic silica beads are coated with a weak cation exchange surface and are ideal for the reduction of protein or peptide complexity. These beads are intended for: 

  • Sample preparation and pre-fractionation prior to mass spectrometry and HPLC 
  • Protein and peptide separation for multiple downstream applications 
  • Detergent removal 
  • Fractionation of clinical samples (serum, plasma, tissues, CSF, urine and cell lysate, etc.)

These beads have the typical ion exchange properties well known from classic chromatography. Protein and peptides are charged at the surface and will be adsorbed to magnetic beads under low salt conditions. Under high salt conditions or by pH shift, the target proteins/peptides are eluted (ion exchanged). 

Magnetic beads coated with a weak cation exchange surface enable easy handling in both manual and automated workflows. The high magnetic strength of the beads typically results in complete collection in less than 1 minute when magnetic force is applied. Fast and complete separation creates strong reproducibility as beads will not be lost during washing steps. Additionally, short incubation times for protein adsorption, desorption and magnetic collection make the process much faster than conventional column-based ion exchange chromatography. 

 

  • Ideal for the reduction of protein or peptide complexity
  • 1.2μm bead size
  • High reproducibility
  • Faster protocol than column-based ion exchange chromatography due to short incubation time
  • Collection in under 1 minute
  • Suitable for use in 96 well microplates on automated liquid handling platforms
  • Can be used in both manual and automated workflows
  • Reduction of protein or peptide complexity
  • Sample preparation and pre-fractionation prior to mass spectrometry and HPLC 
  • Protein and peptide separation for downstream applications
  • Fractionation of clinical samples (serum, plasma, tissues, CSF, urine and cell lysate, etc.)
  • Biomarker analysis
  • Detergent removal 
  • MD01023 - MagSi-WCX beads, 1.2μm bead size, 2ml
  • MD02023 - MagSi-WCX beads, 1.2μm bead size, 10ml