MagSi-Proteomics

MagSi-Proteomics C4, 2ml (Cat# MD01014)
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Sales Price$862.00
Surface 
Amount 
Description

MagSi-Proteomics are magnetic silica beads coated with C4, C8 or C18 alkyl groups. MagSi-Proteomics beads provide a reversed phase surface chemistry and are an ideal tool for protein and peptide sample concentration, desalting and fractionation, and reducing sample complexity.

The three surfaces of MagSi-Proteomics beads differ in their levels of hydrophobicity, making them well-suited for different applications and types of samples:

  • C4 groups - low hydrophobicity; ideal for purification and fractionation of large biomolecules such as proteins
  • C8 groups - intermediate hydrophobicity; suitable for capture and purification of peptides and proteins from urine, saliva and CSF for proteomic profiling and biomarker research
  • C18 groups - high hydrophobicity; ideal for desalting of peptides or protein tryptic digest prior to mass spectrometry, concentration of peptides, and capture and purification of peptides and proteins from serum and plasma

Peptides and proteins bind to MagSi-Proteomics beads via hydrophobic interactions between the protein/peptide and the hydrophobic surface of the beads. The higher the hydrophobic character of the proteins and peptides, the stronger the binding towards the reversed phase surface. Proteins and peptides are eluted under organic solvent conditions and can therefore be separated according to their relative hydrophobicities. This occurs using stepwise desorption in increasing concentrations of organic solvents.

 
  • Magnetic silica particles with reversed phase chemistry on the surface
  • Bead size of 1.2μm
  • Suitable for use in 96 well microplates on automated liquid handling platforms
  • Compatible with common solvents used in mass spectrometry
  • Protein and peptide sample concentration, desalting and fractionation, and reducing sample complexity
  • MagSi-Proteomics C4 are ideal for purification and fractionation of larger biomolecules
  • MagSi-Proteomics C8 are suited for sample preparation
  • MagSi-Proteomics C18 are ideal for purification, concentration and desalting of peptides and protein digests
Cat# Product Surface Volume
MD01014 MagSi-proteomics C4 C4 groups 2ml
MD02014 MagSi-proteomics C4 C4 groups 10ml
MD01015 MagSi-proteomics C8 C8 groups 2ml
MD02015 MagSi-proteomics C8 C8 groups 10ml
MD01009 MagSi-proteomics C18 C18 groups 2ml
MD03009 MagSi-proteomics C18 C18 groups 10ml

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