MobiSpin G-Columns pre-packed with Sephadex® G-50 resin remove small molecules from DNA/RNA, such as salt ions, and are therefore most suitable for buffer exchange.
The Sephadex G-50 matrix is a hydrophilic matrix that swells in buffer and acquires its chromatographic properties under wet conditions. In this state, beads have a pore size of 700 Da and allow hydrated salt ions or dNTPs (dye-labeled or radiolabeled) to enter the pores while DNA/RNA under 20 bases and most other biomolecules stay outside.
The MobiSpin G-Columns are closed with a screw cap and the tip is a fixed snap-off plug.