The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.
The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.
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