Bacillus megaterium Control Vector

pGFP1522 (BMEG10C)
BMEG10C vector map
Sales Price$146.00
Vector 
Please note this product requires a license for commercial companies. Please contact us for details.
Description

The Bacillus megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale. 

Several vectors are available as positive controls for expression in B. megaterium.

  • pGFP1522 (BMEG10C) - expresses GFP under the control of xylose inducible PxylA promoter
  • pRBBm15C (BMEG13C) - encodes protein of levansucrase, HIS-Tag, can be used for secretion
  • pRBBm13C (BMEG14C) - encodes protein of levansucrase, STREP-Tag, can be used for secretion
  • pRBBm16C (BMEG15C) - encodes protein of levansucrase, STREPHIS-Tag, can be used for secretion

These vectors have been validated for recombinant production and one-step affinity purification of L. reuteri levansucrase from a growth medium using a B. megaterium expression system.

Please note positive control vectors are available only in combination with a regular B. megaterium expression vector.

To purchase this product, a license is required for commercial companies.  Please contact us for more information.

 
  • Stable, high-yield protein expression 
  • Ideal for both small- and large-scale protein production
  • Tightly regulated and efficiently inducible xylose operon 
  • No alkaline proteases activity even up to 5 hours after induction
  • No endotoxins observed
  • Positive control for B. megaterium expression system
Cat# Control Vector Encoded Protein Tag
BMEG10C pGFP1522 GFP n/a
BMEG13C pRBBm15C Lev∆773His His-Tag
BMEG14C pRBBm13C StrepLev∆773 Strep-Tag
BMEG15C pRBBm16C StrepLev∆773His StrepHIS-Tag

All vectors are supplied as lyophilized DNA (10µg).

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