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{{/_source.additionalInfo}}NICE® Lactococcus lactis Secretion Vector
pNZ8124 (ELV00800-01)
Sales Price$1,464.00
Please note this product requires a license for commercial companies. Please contact us for details.
Description
The Nisin controlled gene expression system (NICE®), developed at NIZO Food Research, NL, is a fully food-grade system used for controlled gene expression in Lactococcus lactis. The NICE® system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and the tightly controlled gene expression allows the production of toxic proteins to take place. With this system, fermentation, scale-up and downstream processing are simple.
Vectors
- pNZ8120 (ELV00600-01) - broad-host-range vector; CmR; NICE® vector for protein secretion with the signal sequence of the lactococcal cell wall proteinase PrtP; cloning via a NaeI site; mature protein starts with its own first amino acid.
- pNZ8121 (ELV00650-01) - broad-host-range vector; CmR; NICE® vector for protein secretion with the signal sequence of the lactococcal cell wall proteinase PrtP; cloning via an EcoRV site; mature protein starts with the first amino acid of PrtP (Asp) after the signal cleavage site. This setting can contribute to a greater efficiency in removing the signal peptide.
- pNZ8122 (ELV00700-01) - broad-host-range vector; CmR; NICE® vector for protein secretion with the signal sequence of the Lactobacillus brevis SlpA protein; cloning via an NruI site; mature protein starts with the first amino acid of SlpA (Ser) after the signal cleavage site. This setting can contribute to a greater efficiency in removing the signal peptide.
- pNZ8123 (ELV00750-01) - broad-host-range vector; CmR; NICE® vector for protein secretion with the signal sequence of the lactococcal major secreted protein Usp45; cloning via a NaeI site; mature protein starts with its own first amino acid.
- pNZ8124 (ELV00800-01) - broad-host-range vector; CmR; NICE® vector for protein secretion with the signal sequence of the lactococcal major secreted protein Usp45; cloning via an EcoRV site; mature protein starts with the first amino acid (Asp) of Usp45 after the signal cleavage site. This setting can contribute to a greater efficiency in removing the signal peptide.
See also: NICE® Lactococcus lactis Expression Vectors
Please note a license is required for commercial companies. Please contact us for licensing details.
- Endotoxin-free, food-grade expression system
- Ideal for expression of membrane proteins and secretion of proteins into the medium
- Fewer endogenous and no exogenous proteases
- No spores or inclusion bodies
- Tightly controlled gene expression allows production of toxic proteins
- Simple fermentation, scale-up and downstream processing
- Production of homologous and heterologous proteins for food, feed, pharmacological and biocatalysis applications
- Production of prokaryotic and eukaryotic membrane proteins
- Production of exopolysaccharides
- Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
- Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as, e.g., dehydrogenases and in-situ cofactor regeneration
- High-throughput screening for enzyme evolution or enzyme comparison
| Cat# | Vector | Note |
| ELV00600-01 | pNZ8120 | SP PrtP/NaeI |
| ELV00650-01 | pNZ8121 | SP PrtP/EcoRV |
| ELV00700-01 | pNZ8122 | SP SlpA/NruI |
| ELV00750-01 | pNZ8123 | SP Usp45/Nael |
| ELV00800-01 | pNZ8124 | SP Usp45/EcoRV |
All vectors are supplied as lyophilized DNA (10µg).
NICE® Lactococcus lactis Host Strain
NICE® Lactococcus lactis Expression Vector
NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000