This vector is a ready-to-use mammalian expression vector intended for investigation of the human Hec1 complex. It encodes a fusion protein of the fluorescent protein EGFP and human Hec1. These fusion proteins can be used in modern fluorescence microscopy techniques (FRET, FRAP, FCS, RICS, FCCS, and F3H) to determine localization, binding, dynamics, protein-protein interactions and protein-protein proximities. The cipher in the vector name indicates the linker length of the fusion proteins.