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{{/_source.additionalInfo}}Multiple Cloning Site Vector pMCS5
Cat#: PMCS5
Description
The cloning vector pMCS5 contains the ultimate multiple cloning site (MCS) with 59 unique restriction sites. The recognition sites for many commonly used restriction enzymes are included, providing a suitable cloning site for almost any application. This vector contains 46 unique hexamer sites, 2 heptamer sites and the recognition sites for all ten known octanucleotide-specific endonucleases. The well-defined 18-mer sequence of the extremely rarely cutting enzyme I-SceI is located at one terminus of the MCS, enabling the linearization of recombinant clones. I-SceI does not recognize a palindrome and can thus be used for labeling and end-cloning. It can also be used for strand protection in unidirectional deletion experiments. Additionally, the MCS also restriction sites that occur very infrequently in human DNA such as MluI, NruI and SplI.
- MCS has 59 unique restriction sites
- Contains 46 unique hexamer sites, 2 heptamer sites and the recognition sites for all ten known octanucleotide-specific endonucleases
- Includes suitable cloning site for almost any application
- Standard cloning into a site upstream of a T7 RNA polymerase promoter
- End-cloning
- Labeling
- Linearization of recombinant clones
- Blue/white selection
- Generation of single-strand DNA (using the f1 origin)
- pMCS5 Multiple Cloning Site Vector, lyophilized DNA, 10 µg
