NICE® Nisin Kit

(Cat#: ELK01000-02)
l.lactis-image
Sales Price$312.00
Please note this product requires a license for commercial companies. Please contact us for details.
Description

The Nisin controlled gene expression system (NICE®), developed at NIZO Food Research, NL, is a fully food-grade system used for controlled gene expression in Lactococcus Lactis. The NICE® system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and the tightly controlled gene expression allows the production of toxic proteins to take place. With this system, fermentation, scale-up and downstream processing are simple.

The NICE® Nisin kit, part of the NICE® expression system, functions by binding to the lipid A portion of bacterial lipopolysaccharides and then inducing pore formation in the membranes of cortex cells from excised sorghum roots. This kit includes 1 mg/ml pure nisin in 0.05% acetic acid.

Please note this product requires a license for commercial companies. Please contact us for licensing information.

 
  • Endotoxin-free, food-grade expression system
  • Expression of membrane proteins
  • Secretion of proteins into the medium
  • Fewer endogenous and no exogenous proteases
  • No spores or inclusion bodies
  • Tightly controlled gene expression allows production of toxic proteins
  • Simple fermentation, scale-up and downstream processing
  • Production of homologous and heterologous proteins for food, feed, pharmacological and biocatalysis applications
  • Production of prokaryotic and eukaryotic membrane proteins
  • Production of exopolysaccharides
  • Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
  • Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as, e.g., dehydrogenases and in-situ cofactor regeneration
  • High-throughput screening for enzyme evolution or enzyme comparison
  • 1 mg/ml pure nisin in 0.05% acetic acid

Recently Viewed Products