Home New Customer? Create Account
Member Login:

DNA Polymerases/SNPase

108220 - SNPase DNA Polymerase, Hotstart, 20 U/µl

PRICE

Starting at: $135.00

Please Choose:

Size



Add to Cart:
SNPase HotStart DNA Polymerase is the HotStart version of Klen SNPase. It is designed for cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing as well as for manual DNA sequencing with 35S label. This enzyme has only 5 ‘- 3’ polymerase activity and is also recommended for SNP genotyping by allele-specific PCR (AS-PCR), allele-specific primer extension (AS-PEX) and mini-sequencing procedures. The relative mutation rate during polymerization is 10-15 fold lower for Klen SNPase compared with Taq DNA polymerase.
 
 

Features

  • High fidelity of dNTPs and ddNTPs incorporation
  • High specificity leads to low level of background PCR
 

Applications

  • Cycle sequencing with dideoxynucleotides in automated fluorescent DNA sequencing or manual DNA sequencing
  • SNP genotyping with AS-PCR, AS-PEX and minisequencing procedures
 

Sizes  

 
108210 (500 units)
  • SNPase HotStart DNA Polymerase, 20 U/µl, 500 units
  • Incomplete Reaction Buffer (10x), 1.25ml
  • MgCl2 100 mM, 1ml
108210 (2500 units)
  • SNPase HotStart DNA Polymerase, 20 U/µl, 2500 units
  • Incomplete Reaction Buffer (10x), 1.25ml x 2
  • MgCl2 100 mM, 1ml x 2

 

Literature