Anti-DRAM1 [M3-P4B4]

(Cat#: 151871)
Description
Cat# 151871
Applications ELISA, IHC, IF, WB
Antigen/Gene or Protein Targets Damage Regulated Autophagy Modulator 1 (DRAM1)
Reactivity Human
Relevance DRAM1 is significantly upregulated in colorectal cancer and significantly associated with prognosis.
Host Mouse
Immunogen Peptide Sequence - EKDYVYHVVS (amino acids 192 - 201)
Positive Control

ELISA: Peptide immunogen

Western Blot: Hela cell extract (cytoplasmic fraction)

IF: Hela Cells

IHC: Human hepatocellular carcinoma

Subclass IgG1
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin.
Strain Balb/c
Research Area Apoptosis and Programmed Cell Death, Cancer, Cardiovascular, Cell Cycle, Cell Signaling & Signal Transduction, Metabolism
References The expression and prognostic significance of retinoic acid metabolising enzymes in colorectal cancer. Europe PMC ID: 24608339

Image: Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Positive cytoplasmic staining is observed in hepatocellualr carcinoma using anti-DRAM1 antibody [M3-P4B4]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0)

For Research Use Only

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