Anti-NSE [NSEP2]

(Cat#: 151867)
Description
Cat# 151867
Applications ELISA, IHC, WB
Antigen/Gene or Protein Targets Gamma-isozyme of human enolase (NSE)
Reactivity Human
Relevance Neuron specific enolase (NSE) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
Host Mouse
Immunogen Immunogen: ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P2: aa’s 271-285 - TGDQLGALYQDFVRD
Positive Control

Formalin-fixed, paraffin-embedded nerve tissue sections

Western blot: isozyme of human enolase; 50-100 ng per lane

Subclass IgG1 kappa
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine + 3ml of Penicillin -Streptomycin.
Strain Balb/c
Notes Not reactive with -isozyme of enolase.
Research Area Cancer, Cell Type or Organelle Marker, Developmental Biology, Metabolism, Neurobiology, Stem Cell Biology
References

Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies. Europe PMC ID: 8254105

A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific gamma isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences. Europe PMC ID: 1629611

For Research Use Only. 

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