Cat# | 152125 |
Applications | ELISA, IHC, WB |
Antigen/Gene or Protein Targets | RYK receptor-like tyrosine kinase |
Reactivity | Human, Mouse and Rat |
Relevance | RYK receptor-like tyrosine kinase may be a co-receptor of Wnt proteins such as WNT1, WNT3, WNT3A and WNT5A. RYK is involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. |
Host | Mouse |
Immunogen | Peptide sequence - CLTEFHAALGAYV |
Positive Control | Western Blot - E.coli expressed RYK protein, HCT116 cell lysates and Jurkat cell lysates |
Subclass | IgG1 kappa |
Molecular Weight (kDa) | 68 |
Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine). |
Strain | Balb/c |
Notes | BLAST analysis has revealed the immunogen peptides is 100% identity to all vertebrates (mammals, birds, reptiles, amphibians and fishes |
Research Area | Cell Signaling & Signal Transduction, Stem Cell Biology |
Image: Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Staining of normal human liver using Anti-RYK [F35P7D7F5] shows positive cytoplasmic and membranous immuno-reactivity. Antigen retrieval step is required using EDTA buffer, PH8.0
For Research Use Only