Anti-CD3 [T1P1D4*C6]

(Cat#: 153303)
Description
Cat# 153303
Applications ELISA, FACS, IHC, WB
Antigen/Gene or Protein Targets T-cell surface glycoprotein CD3 gammadelta-A chain
Synonyms CD3, CD3gd, cd3g/d, cd3-gamma
Reactivity Salmon and Rainbow Trout
Relevance Anti-CD3 [T1P1D4*C6] is specific for CD3 in Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss).
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY
Positive Control

Western Blot: Spleen lysates

IHC: Formalin-fixed, paraffin-embedded PKD infected kidney of rainbow trout

FACS: Spleen cells, peripheral blood lymphocyte (PBL)

Subclass IgG1 kappa
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes

Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY, 91% sequence identity with large yellow croaker (Larimichthys crocea), Sablefish (Anoplopoma fimbria), 82% sequence identity with fugu (Takifugu rubripes), snappers (Lutjanus sanguineus).

Recommended Usage Conditions: ELISA - neat; Western Blot - neat; IHC - 1/3 dilution (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0); Flow cytomtery - 1/4-1/8 dilution (cells were fixed and permeabilized by adding 100 µl of BD Cytofix/Cytoperm to 1 × 105 lymphocytes for 15 min)

Research Area Aquaculture, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology
References Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. Veenstra et al. 2019. Fish Shellfish Immunol. 87:559-564. PMID: 30731214

Image: Immunohistochemistry image showing positive membranous staining of Anti-CD3 [T1P1D4*C6] in vaccinated rainbow trout head kidney. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0). 

For Research Use Only.

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