Cat# | 153303 |
Applications | ELISA, FACS, IHC, WB |
Antigen/Gene or Protein Targets | T-cell surface glycoprotein CD3 gammadelta-A chain |
Synonyms | CD3, CD3gd, cd3g/d, cd3-gamma |
Reactivity | Salmon and Rainbow Trout |
Relevance | Anti-CD3 [T1P1D4*C6] is specific for CD3 in Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). |
Host | Mouse |
Immunogen | Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY |
Positive Control |
Western Blot: Spleen lysates IHC: Formalin-fixed, paraffin-embedded PKD infected kidney of rainbow trout FACS: Spleen cells, peripheral blood lymphocyte (PBL) |
Subclass | IgG1 kappa |
Myeloma Used | P3X63Ag8.653 |
Strain | Balb/c |
Notes |
Ovalbumin-conjugated synthetic peptide- TLEYKDENTNEY, 91% sequence identity with large yellow croaker (Larimichthys crocea), Sablefish (Anoplopoma fimbria), 82% sequence identity with fugu (Takifugu rubripes), snappers (Lutjanus sanguineus). Recommended Usage Conditions: ELISA - neat; Western Blot - neat; IHC - 1/3 dilution (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0); Flow cytomtery - 1/4-1/8 dilution (cells were fixed and permeabilized by adding 100 µl of BD Cytofix/Cytoperm to 1 × 105 lymphocytes for 15 min) |
Research Area | Aquaculture, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology |
References | Immunohistochemical examination of immune cells in adipose tissue of rainbow trout (Oncorhynchus mykiss) following intraperitoneal vaccination. Veenstra et al. 2019. Fish Shellfish Immunol. 87:559-564. PMID: 30731214 |
Image: Immunohistochemistry image showing positive membranous staining of Anti-CD3 [T1P1D4*C6] in vaccinated rainbow trout head kidney. IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).
For Research Use Only.