Anti-IFN-y [N2P2D5*A10]

(Cat#: 152858)
Description
Cat# 152858
Applications ELISA, FACS, WB
Antigen/Gene or Protein Targets Interferon-gamma (IFNγ)
Synonyms IFG, IFI, IFN gamma, IFN, immune, IFN-gamma, IFNG, IFNG_HUMAN, Immune interferon, Interferon gamma
Reactivity Salmon and Rainbow Trout
Relevance The zebrafish genome contains ten genes that encode class II cytokine-like peptides, of which the two that are related most closely to mammalian interferon gamma (IFN-gamma) were named IFN-gamma1 and IFN-gamma2.
Host Mouse
Positive Control

Western blot: recombinant IFNγ and kidney lysates

FACS: isolated leukocytes, kidney and spleen cells

Subclass Not known
Myeloma Used P3X63Ag8.653
Strain Balb/c
Research Area Aquaculture

Image: Recognition of trout IFN-γ within head kidney cells stimulated with trout rIL-2 in vitro. Samples were lysed in Tri-Reagent and RNA and protein were extracted for analysis. Results from qPCR (A) and WB (B) were correlated, and 72-h incubation of HK cells with rIL-2 showed a high level of IFN-γ.

For Research Use Only.

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