Anti-IgM [BC]

(Cat#: 153308)
Description
Cat# 153308
Applications ELISA, FACS, IHC, IP, WB
Antigen/Gene or Protein Targets Immunoglobulin Heavy Constant Mu (IgM)
Synonyms IgM, IgM heavy chain constant region antibody, Immunoglobulin mu antibody, Constant region of heavy chain of IgM antibody, Immunoglobin heavy chain mu constant region antibody
Reactivity Salmon and Rainbow Trout
Relevance Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. Moreover, our anti-IgM antibody provides a useful tool to monitor vaccine performance in fish and will assist in the: development of future vaccines.
Host Mouse
Immunogen Ammonium sulphate precipitated rainbow trout Ig fraction
Positive Control

ELISA, IP, WB: fish sera

FACS: peripheral blood lymphocyte, spleen, kidney

IHC: PKD infected kidney of rainbow trout.

Subclass IgG1
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes

Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20°C. Avoid repeated freeze/thaw cycles.

Points of Interest
Recommended Usage Conditions: ELISA 1/3 dilution,; Western Blot neat; IHC 1/5 dilution; IP neat; FACS neat. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).

Concentration
1mg/ml as standard

Research Area Aquaculture

Image: Immunohistochemistry image using Anti-IgM [BC] showing positive membranous and diffuse immunostaining in infected trout kidney. Formalin-fixed paraffin-embedded tissues. 

For Research Use Only. 

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