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{{/_source.additionalInfo}}Anti-3-nitroY | Nitrotyrosine
(Cat#: AS10 706-100)
Description
- Immunogen: BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid
- Host: Mouse
- Clonality: Monoclonal
- Purity: Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
- Format: Liquid
- Quantity: 100 µg
- Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: 1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
- Confirmed reactivity: Dog, Human, Mouse, Rat
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
- Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
- The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific.0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.