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{{/_source.additionalInfo}}Anti-RE | N-terminal arginylation
(Cat#: AS15 3050)
Description
- Immunogen: KLH-conjugated synthetic peptide: H-REHKHANQHMSVC-NH2
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 2x50 µg
- Reconstitution: For reconstitution add 50 µl of sterile water to each tube
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Dot blot (Dot)
- Recommended dilutions: 1 : 1000 (Dot)
- Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.
- MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins
