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{{/_source.additionalInfo}}Anti-VDE | Violaxanthin de-epoxidase
(Cat#: AS15 3091)
Description
- Immunogen: His-tagged, recombinant VDE from Arabidopsis thaliana, overexpressed in E.coli, Uniprot:Q39249 , TAIR: AT1G08550
- Host: Rabbit
- Clonality: Polyclonal
- Purity: Serum
- Format: Lyophilized
- Quantity: 50 µl
- Reconstitution: For reconstitution add 200 µl of sterile water
- Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Western blot (WB)
- Recommended dilutions: 1 : 2000 (WB)
- Expected | apparent MW: Propeptide 52 kDa, mature protein: 39.7 kDa, migrates at 40 kDa (Arabidopsis thaliana)
- Confirmed reactivity: Arabidopsis thaliana, Brachypodium distachyon, Deschampsia antarctica, Oryza sativa
- Not reactive in: Diatoms
- VDE (violaxanthin de-epoxidase) is a part of the xanthophyll (or violaxanthin) cycle for controlling the concentration of zeaxanthin in chloroplasts. This enzyme is catalyzing the two-step mono de-epoxidation reaction. Zeaxanthin induces the dissipation of excitation energy in the chlorophyll of the light-harvesting protein complex of photosystem II.
- Extraction buffer must contain 7 M urea for Brachypodium distachyon and Oryza sativa samples.This product can be sold containing ProClin if requested.