Anti-H3K9me2 | Histone H3 dimethylated lysine 9

(Cat#: AS16 3194)
Description
  • Immunogen: KLH-conjugated synthetic peptide
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum.
  • Format: Liquid
  • Quantity: 50 µg
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Chromatin Immunoprecipitation (ChIP), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
  • Recommended dilutions: 1-5 µg/IP (ChIP/ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
  • Expected | apparent MW: 15 kDa
  • Confirmed reactivity: Human, Solanum lycopersicum
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.
  • Liu et al. (2018). Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.
  • Antibody solution contains PBS with 0,05% sodium azide and 0,05% ProClin 300