Anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)

(Cat#: AS16 3195)
Description
  • Immunogen: KLH-conjugated synthetic peptide
  • Host: Rabbit
  • Clonality: Polyclonal
  • Purity: Immunogen affinity purified serum.
  • Format: Liquid
  • Quantity: 50 µg
  • Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Tested applications: Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
  • Recommended dilutions: 0.5-5 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 500 (ELISA), 1 : 500 (IF), 1 : 1000 (WB)
  • Expected | apparent MW: 15 kDa
  • Confirmed reactivity: Human
  • Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
  • Antibody solution contains PBS with 0.05% sodium azide and 0.05% ProClin 300