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{{/_source.additionalInfo}}Anti-WHIRLY1
(Cat#: AS16 3953)
Description
- Immunogen: KLH-conjugated peptide derived from HvWHIRLY1 protein sequence, NCBI
- Host: Goat
- Clonality: Polyclonal
- Purity: Immunogen affinity purified serum in PBS pH 7.4.
- Format: Lyophilized
- Quantity: 50 µg
- Reconstitution: For reconstitution add 50 µl, of sterile water
- Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunogold (IG)
- Recommended dilutions: 1: 200 (IG)
- Confirmed reactivity: Hordeum vulgare
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).
- Karpinska et al. (2022). WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles. Biochem J. 2022 Mar 18;479(5):641-659. doi: 10.1042/BCJ20210810. PMID: 35212355; PMCID: PMC9022988.