Immunogen: Synthetic peptide derived from the N-terminal of the human beta-tubulin
Host: Mouse
Clonality: Monoclonal
Purity: IgG2a purified on Protein A n 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format: Lyophilized
Quantity: 100 µg
Storage: Store at -20°C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°C for several days to weeks
Tested applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilutions: 1: 2000 - 1: 5000 (WB), for other techniques end user needs to determine most optimal diltion
Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.