Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format: Liquid at 2 mg/ml.
Quantity: 100 µg
Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: Immunofluorescence (IF), Western blot (WB)
Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds. May also be implicated in targeting N-terminal propeptide containing proteins to lytic vacuoles. Subcellular localisation: Golgi apparatus membrane.Alternative names: BP80-like protein b, Epidermal growth factor receptor-like protein 1, AtELP, AtELP1, Spot 3 protein
Fuji et al. (2016). The Adaptor Complex AP-4 Regulates Vacuolar Protein Sorting at the trans-Golgi Network by Interacting with VACUOLAR SORTING RECEPTOR1. Plant Physiol. 2016 Jan;170(1):211-9. doi: 10.1104/pp.15.00869. (Western blot, Arabidopsis thaliana) Kunieda et al. (2013). Spatiotemporal secretion of PEROXIDASE36 is required for seed coat mucilage extrusion in Arabidopsis. Plant Cell. 2013 Apr;25(4):1355-67. doi: 10.1105/tpc.113.110072. (Western blot, Arabidopsis thaliana)Yamada et al. (2005). Endosomal proteases facilitate the fusion of endosomes with vacuoles at the final step of the endocytotic pathway. Plant J. 2005 Mar;41(6):888-98. doi: 10.1111/j.1365-313X.2005.02349.x. (Immunofluorescence, Nicotiana tabacum)