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{{/_source.additionalInfo}}Anti-Tubulin gamma chain (monoclonal antibodies)
(Cat#: AS20 4482)
Description
- Immunogen: KLH-conjugated gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449. UniProt: P23258The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
- Host: Mouse
- Clonality: Monoclonal
- Purity: Immunoglobulin Protein A purified in PBS. Contains 15 mM sodium azide.
- Format: Liquid
- Quantity: 100 µg
- Storage: Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Immunocytochemistry (ICC), Western blot (WB)
- Recommended dilutions: 2-8 µg/ml (ICC) methanol/acetone fixation required, 1-2 µg/ml (WB)
- Expected | apparent MW: 51 kDa
- Confirmed reactivity: Chlorella vulgaris, Nicotiana tabacum, human, protozoa
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Tubulin gamma chain is essential for the control of microtubular network. It is found at microtubule organizing centers (MTOC) such as the spindle poles
- Romeiro Motta et al. (2024). The cell cycle controls spindle architecture in Arabidopsis by activating the augmin pathway. Dev Cell. 2024 Aug 23:S1534-5807(24)00484-2. doi: 10.1016/j.devcel.2024.08.001.
- This antibody recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope which this antibody is recognizing is conserved in Arabidopsis thaliana Tubulin gamma-1 chain, UniProt: P38557, Gene ID: At3g61650 and Tubulin gamma-2 chain, UniProt: P38558, Gene ID: At5g05620Recommended secondary antibody: goat anti-mouse IgG1 AS16 3715