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{{/_source.additionalInfo}}Anti-Tubulin alpha chain (monoclonal antibody)
(Cat#: AS20 4483)
Description
- Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization, UniProt: Q71U36
- Host: Mouse
- Clonality: Monoclonal
- Purity: Immunoglobulin Protein A purified in PBS. Contain 15 mM sodium azide.
- Format: Liquid
- Quantity: 100 µg
- Storage: Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: Flow cytometry (FlowCyt), Immunocytochemistry (ICC), Immunohistochemistry on paraffin sections (IHC), Immunoprecipitation (IP), Western blot (WB)
- Recommended dilutions: 1 : 500 (ICC), 1-4 µg/ml /FlowCyt), 1-4 µg/ml (WB)
- Expected | apparent MW: 51 kDa
- Confirmed reactivity: Arabidopsis thaliana, Chlorella vulgaris, Eisenia, human, mouse, Nicotiana tabacum, Paramecium sp. , pig, turkey, yeast
- Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
- Liu et al. (2022) Identification of positive and negative regulators of antiviral RNA interference in Arabidopsis thaliana. Nat Commun. 2022 May 30;13(1):2994. doi: 10.1038/s41467-022-30771-0. PMID: 35637208; PMCID: PMC9151786.
- This antibody is recognizing defined epitope (amino acid 65-97) on N-terminal structural domain of alpha tubulin.Recommended secondary antibody, goat anti-mouse IgG1, HRP conjugated AS16 3715