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{{/_source.additionalInfo}}Anti-BFP, GFP, YFP | Fluorescent Protein, clone 3A6
(Cat#: AS20 4485)
Description
- Immunogen: Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
- Host: Mouse
- Clonality: Monoclonal
- Purity: Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
- Format: Lyophilized
- Quantity: 100 µg
- Reconstitution: Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05%) is recommended for long term storage. •For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
- Storage: Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
- Tested applications: ELISA (ELISA), Western blot (WB)
- Recommended dilutions: 1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)
- Confirmed reactivity: BFP, GFP, YFP
- Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.