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{{/_source.additionalInfo}}Goat anti-Human IgM (µ chain), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to human IgG or IgA
(Cat#: AS10 959)
Description
- Host: Goat
- Clonality: Polyclonal
- Purity: Immunogen affinity purified goat IgG, F(ab)'2 fragment.
- Format: Lyophilized
- Quantity: 0.5 mg
- Reconstitution: For reconstitution add 0.55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
- Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
- Recommended dilutions: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
- Goat anti-human IgM, µ chain, F(ab)'2 fragment is a HRP conjugated secondary antibody which is reacting with human IgM, µ chain, F(ab)'2. Min. cross-reactivity to human IgG or IgA. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
- This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG or IgA immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10% (w/v) BSA, Protease/IgG free and 0.1% (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
