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{{/_source.additionalInfo}}BriClone Hybridoma Cloning Additive
Cat#: BRI100
Description
Additive for increased efficiency in hybridoma production.
BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma cell cloning. It has been specifically developed by the NICB's dedicated team to overcome common problems in the post-fusion period, such as batch-to-batch variation of feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium.
BriClone is intended for use when cloning hybridoma cell lines under conditions of limiting dilution. This conditioned medium was collected from a proprietary human cell line that produces IL-6. Each batch is tested for its ability to support the outgrowth of hybridomas from freshly fused cells and for its ability to support the growth of hybridomas under conditions of limiting dilution.
To use BriClone, add to your hybridoma culture media as a 5% (v/v) supplement. Use of this product eliminates the need for feeder cells in these types of cultures.
Figure 1: BriClone significantly increased the number of positive wells identified with hybridoma clones in comparison with two competitor products. Learn more
- Increases efficiency in hybridoma production
- Eliminates need for feeder cells; avoids time-consuming preparation of feeder cells and depletion of nutrients by feeder cells
- Prevents batch variation
- Screened for mycoplasma contamination
- Intended for use when cloning under conditions of limiting dilution
- Supplied in a sterile frozen format in 100ml bottles
- Hybridoma cloning
- Hybridoma growth post-fusion
- BriClone Hybridoma Cloning Additive, 100ml
Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.
