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PCR & RNA Extraction

RQ2110 - ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX), 200 Reactions

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The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. 

This kit includes One-Step RT Enzyme Mix for efficient cDNA synthesis and 2X One-Step qPCR Master Mix for highly specific real-time PCR in convenient, single tube formats.

The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. With these components, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range of 42-60°C. It is active against RNase A, RNase B and RNase C. 

The 2X One-Step qPCR Master Mix includes a specialized Hot-Start Taq DNA polymerase that greatly reduces primer-dimer formation and can be activated within 2 minutes. This master mix has high specificity and is suitable for fast cycle programs.

This product includes ROX reference dye for normalization of each RT-qPCR assay. A version without ROX is also available.

 

Features

  • High yield and specificity
  • Performs reverse transcription at wide temperature range (42-60°C)
  • Suitable for fast cycle programs
  • Convenient single tube format
  • Includes ROX reference dye

 

Applications

  • RT-qPCR

 

Contents

  • One-Step RT Enzyme Mix, 400μl
  • 2X One-Step qPCR Master Mix, 1ml x 2

 

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