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Saliva Collection & Sample Stabilization

7030 - AquaStool Solution, 30ml

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$225.00

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AquaStool is a multifunctional aqueous reagent for fecal sample stabilization, DNA and RNA extraction and PCR inhibitor removal. It is used to extract fecal DNA for non-invasive genotyping of transgenic animals and to preserve and extract DNA/RNA from human stools for host and gut microbiome research. AquaStool can also be used to extract DNA from tree tissues, which are particularly challenging with existing DNA extraction methods.

Three separate protocols are provided:

  • Mouse fecal DNA extraction and genotyping
  • Human fecal DNA/RNA extraction and analysis 
  • Wood DNA extraction
     

Please note: AquaRemove reagent is required for human fecal DNA/RNA purification. This reagent is purchased separately.

 

Features

  • Non-invasive sampling
  • Three protocols provided
  • All-in-one solution

 

Applications

  • Fecal sample stabilization, DNA/RNA extraction and PCR inhibitor removal
  • DNA extraction from wood tissue

 

Contents

  • AquaStool Reagent, 30ml (200 extractions on mouse fecal pellets)

 

Literature

 

Citations

  • Al-Masaudi S, El Kaoutari A, Drula E, Redwan EM, Lombard V, Henrissat B. A metagenomics investigation of carbohydrate-active enzymes along the goat and camel intestinal tract. Int Microbiol. 2019;22(4):429-435. doi:10.1007/s10123-019-00068-2
  • Chen Z, Mantha RR, Chen JS, Slivano OJ, Takahashi H. Non-invasive genotyping of transgenic animals using fecal DNA. Lab Anim (NY). 2012;41(4):102-107. Published 2012 Mar 20. doi:10.1038/laban0412-102
  • Deakin S, Gorrell J, Kneteman J, Hik D, Jobin R, Coltman D. Spatial genetic structure of Rocky Mountain bighorn sheep (Ovis canadensis canadensis) at the northern limit of their native range. Can J Zool. 2020;98(5):317-330. doi:10.1139/cjz-2019-0183
  • Frémont M, Coomans D, Massart S, De Meirleir K. High-throughput 16S rRNA gene sequencing reveals alterations of intestinal microbiota in myalgic encephalomyelitis/chronic fatigue syndrome patients. Anaerobe. 2013;22:50-56. doi:10.1016/j.anaerobe.2013.06.002
  • Hunt PW, Lello J. How to make DNA count: DNA-based diagnostic tools in veterinary parasitology. Vet Parasitol. 2012;186(1-2):101-108. doi:10.1016/j.vetpar.2011.11.055
  • Millan BT. Fecal Microbial Transplantation and its Expansion into the Treatment of Other Diseases. University of Alberta.
  • Rajaruban S. The Role of High Sugar Diets in Colitis. University of Alberta.
  • Zhang Z, Hyun JE, Thiesen A, et al. Sex-Specific Differences in the Gut Microbiome in Response to Dietary Fiber Supplementation in IL-10-Deficient Mice. Nutrients. 2020;12(7):2088. Published 2020 Jul 15. doi:10.3390/nu12072088