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DNA Loading Dyes and Stains

TF3000 - G-HiFi DNA Polymerase, (1 U/ul, 100 U)

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Starting at: $110.00

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G-HiFi DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. 

The fidelity of G-HiFi DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. 

The optimized 5X G-HiFi Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi Buffer, G-HiFi DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and targets that contain GC-rich sequences. 

The storage buffer is composed of 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol.

 

Features

  • 5’→3’ DNA polymerase activity 
  • 3’→5’ exonuclease (proofreading) activity 
  • High reaction rate = 7 seconds/kb 
  • High fidelity = 70 times higher than Taq polymerase 
  • Generates blunt end amplicons  
  • Vast elongation capability (up to 40 kb) 
  • Thermo-stable for more than 10 hours at 95°C 

 

Applications

  • Long range PCR amplification
  • PCR for DNA sequencing
  • Amplification of GC-rich templates

 

Contents

  • G-HiFi DNA Polymerase, 100 μl
  • 5X G-HiFi Buffer, 1200 μl
  • dNTPs Mix (2 mM each), 600 μl