DNA Loading Dyes and Stains

G-HiFi DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. 

The fidelity of G-HiFi DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. 

The optimized 5X G-HiFi Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi Buffer, G-HiFi DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and targets that contain GC-rich sequences. 

The storage buffer is composed of 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol.

Features

• 5’→3’ DNA polymerase activity 
• 3’→5’ exonuclease (proofreading) activity 
• High reaction rate = 7 seconds/kb 
• High fidelity = 70 times higher than Taq polymerase 
• Generates blunt end amplicons  
• Vast elongation capability (up to 40 kb) 
• Thermo-stable for more than 10 hours at 95°C 

Applications

• Long range PCR amplification
• PCR for DNA sequencing
• Amplification of GC-rich templates

Contents

• G-HiFi DNA Polymerase, 100 μl
• 5X G-HiFi Buffer, 1200 μl
• dNTPs Mix (2 mM each), 600 μl