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DNA Polymerases/SNPase, dNTPs & Kits

401001 - Cot I Human DNA, 500µg

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COT I Human DNA is prepared exclusively from male human placental DNA by shearing, denaturing, and reannealing under conditions that enrich repetitive elements. The COT I fraction of human genomic DNA consists largely of rapidly annealing repetitive elements.

COT I Human DNA can be used for suppressing cross hybridization to human repetitive DNA in filter and microarray hybridizations and in situ hybridization experiments. Repetitive elements present in a probe may result in unspecific hybridization signals. To enable specific hybridization of the probe to the chromosomal target site, the probe is denatured together with an excess of unlabeled COT I Human DNA as a competitor. As a result, single-copy gene hybridization is not hindered by repetitive sequences.

 

Features

  • Acts as a blocking agent for repetitive sequences
  • Decreases false positives in Microarrays, In situ hybridizations and Southern blots
  • Prepared from human placenta DNA, male
  • Balanced proportion of Y-chromosomal sequences 
  • Enriched repetitive sequences > 98% 
  • Reliable size distribution 50 – 300 bp 
  • Consistent batches
  • Virus-free, phenol treated

 

Applications

  • Filter/ Microarray hybridization assays
  • In-situ hybridization
  • Single-copy-gene hybridization
  • Reduction of cross hybridization

 

Contents

  • Cot I Human DNA, 500µg