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DNA Polymerases/SNPase, dNTPs & Kits

108105/108125 - DF-Pfu DNA Polymerase

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Starting at: $305.00

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DF-Pfu DNA Polymerase is a thermostable polymerase of approximately 92 kDa isolated from the hyperthermophilic archae bacteria Pyrococcus furiosus

This enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5’ → 3’ direction in the presence of magnesium with a preference for MgSO4. DF-Pfu DNA Polymerase is free of bacterial DNA.

Unlike Taq DNA Polymerase, DF-Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. Due to this feature, DF-Pfu DNA Polymerase-generated PCR fragments exhibit the lowest error rate of any thermostable DNA polymerase. This results in a 12-fold increase in fidelity of DNA synthesis compared with Taq DNA Polymerase, which has an error rate Pfu 1.3 x 10-6.

 

Features

  • 3’ to 5’ exonuclease proofreading activity
  • Generates blunt-ended DNA-fragments
  • Free of bacterial DNA

 

Applications

  • PCR and primer extension reactions that require high-fidelity synthesis
  • Blunt-end ligation
  • Precise amplification of bacterial sequences

 

Sizes

108105 (500 U)

  • DF-Pfu DNA Polymerase, 5 U/µl, 500 U
  • Incomplete Reaction Buffer (10x), 1.8ml
  • Complete Reaction Buffer (10x), 1.8ml
  • MgSO4 100 mM, 1ml

108125 (2500 U)

  • DF-Pfu DNA Polymerase, 5 U/µl, 2500 U
  • Incomplete Reaction Buffer (10x), 7.2ml
  • Complete Reaction Buffer (10x), 7.2ml
  • MgSO4 100 mM, 4ml