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Culture Media

GCM09.0500 - 2xYT Medium, 500g

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2xYT Medium is a dehydrated powder for the preparation of nutritionally rich liquid medium for the growth and maintenance of recombinant strains of E. coli in molecular biology studies, as well as for the growth of filamentous phage.

This rich growth medium is optimized for the growth and maintenance of filamentous phage such as M13. It contains all the nutritional requirements for E. coli. Tryptone and Yeast Extract are the sources for carbon, nitrogen, vitamins, minerals, and amino acids essential for growth, as well as growth factors that allow phages to reproduce without weakening the host cells. Sodium chloride supplies essential electrolytes for transport and osmotic balance. 

 
Formulation (g/l)
  • Tryptone: 16.00
  • Yeast Extract: 10.00
  • NaCl: 5.00
  • Final pH (25°C): 7.0 ± 0.2
 

Features

  • Optimized for growth and maintenance of filamentous phage such as M13 
  • Contains all the nutritional requirements for E. coli
  • Growth factors allow phages to reproduce without weakening the host cells
  • Prepares liquid medium
 

Contents

  • 2xYT Medium, 500g