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{{/_source.additionalInfo}}Zymolyase® 20T
Cat#: GE013.0001
Description
Zymolyase® 20T is prepared from Arthrobacter luteus and is supplied as an ammonium sulfate precipitate of a complex of enzymes in the form of a lyophilized powder. The strong lytic activity against living yeast cell walls is mainly due to the activity of ß-1,3-glucan laminaripentaohydrolase. This enzyme hydrolyzes linear glucose polymers with β-1,3-linkages and releases laminaripentaose as the main and minimum product unit. This allows the production of protoplasts or spheroplasts of various yeast strains.
The lytic activity of Zymolyase® 20T varies depending on the yeast strain, growth stage of the yeast, or cultural conditions. Under the conditions described below, the lytic activity of Zymolyase® 20T is 20 U/g.
One unit (U) of lytic activity is defined as the amount that results in a 30% decrease of the absorbance at 800nm of a reaction mixture* after incubation with gentle shaking at 25ºC for 2 hours. A decrease of A800 with 60%, equivalent to 2U, corresponds to complete lysis. In other words, 1U of Zymolyase® lyses 3mg of dry weight Saccharomyces cerevisiae in 2 hours under ideal conditions.
*10ml reaction mixture is composed of 1 ml of distilled water, 3 ml of S. cerevisiae (2mg/ml), 5 ml 1/15 M phosphate buffer (pH 7.5), and 1 ml of enzyme solution.
- Form: Lyophilized powder
- Purification: Ammonium Sulfate Precipitation
- Activity: ß-1.3-glucan laminaripentaohydrolase (20 U/g)
- Other activities: ß-glucanase (1.5x106 U/g), protease (1.0x104 U/g), mannanase (1.0x106 U/g)
- Contaminants: trace amounts of amylase and xylanase
- Optimum conditions for lysis of viable yeast cells: pH 7.5, 35℃
- Optimum conditions for hydrolysis of yeast glucan: pH 6.5, 45℃
- Preparation of spheroplasts or protoplasts from a variety of yeast strains
- Zymolyase® 20T, lyophilized powder, 1g
