The phagemid pSKAN8 is a phagemid vector used to prepare your own phagemid display library. pSKAN8 encodes an hPSTI-pIII fusion protein, a specific inhibitor of the protease trypsin. pSKAN8 was used for the construction of the pSKAN phagemid libraries HyA, HyB, and HyC found in Mobitec’s pSKAN Phagemid Display System. A protocol is provided for preparation of your own library. This protocol was designed for the presentation of a hypervariable amino acid loop of 6-8 amino acids.