Xpert One-Step Fast Probe uses gene-specific primers to perform first-strand cDNA synthesis and subsequent qPCR.
The kit consists of a RTase mix, a Fast qPCR Master mix (Probe), and a 100x ROX reference dye. The reaction is completed in a single tube, reducing handling time and decreasing contamination risks. Xpert One-Step Fast Probe can be used to quantify virtually any RNA target, including extremely low-copy number targets, regardless of the type of template (mRNA, viral RNA, total RNA).
The RTase mix comprises a genetically modified thermostable MMLV reverse transcriptase and an advanced RNase inhibitor to impede RNA degradation. The transcriptase improves synthesis efficiency as it lacks any RNase H activity.
The Fast qPCR Master mix (Probe) consists of a highly efficient enzyme with a novel low inhibitory technology. This combination prevents the formation of unwanted primer-dimers and non-specific products, resulting in high sensitivity and specificity. This master mix can be used in qPCR in a wide range of probe-based technologies, including Taqman®, Molecular Beacons® and Scorpion probes®.
Xpert One-Step Fast Probe works well with the recommended primers/probes from the WHO for COVID-19 research. Due to high demand, this kit is now available as a single solution in bulk format. Larger sizes are available. Please contact us for details.