2x PCR Master Mix Solution (i-Taq™) is ready to use with all components required for a PCR reaction contained in the solution. The 2x master mix solution includes i-Taq™ DNA Polymerase for high performance.
i-Taq™ DNA Polymerase is a thermostable DNA polymerase of 94 kDa, which is expressed in E. coli by cloning the polymerase gene of Thermus aquaticus (strain YT1). This Polymerase has high purity due to the removal of E. coli-derived proteins and DNA that may act as PCR sources. DNA amplification up to 5 Kb from genomic DNA and cDNA can be achieved.
With 2x PCR Master Mix Solution (i-Taq™), PCR can be performed immediately by simply adding the template DNA, a primer set and D.W. to the solution. Gel loading buffer is included in the master mix, so further treatment is not required. The optimized buffer composition creates the best polymerase activity regardless of the template type or reaction conditions. PCR with 2x PCR Master Mix Solution (i-Taq™) is fast and efficient with highly reproducible results.